Sodium dodecyl sulfate-polyacrylamide gel typing system for characterization of Neisseria meningitidis isolates
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چکیده
منابع مشابه
High-temperature sodium dodecyl sulfate polyacrylamide gel electrophoresis.
The run time for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) can be reduced to as little as 5 min by running vertical slab gels at 70°C. High-temperature SDS-PAGE increases the migration rate of protein bands several fold and is as effective as cooling in preventing uneven migration rates across the width of the gel. Additional benefits of hightemperature SDS-PAGE inclu...
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Thirty-seven Borrelia burgdorferi strains, isolated in 1992 from Ixodes ricinus in Finland, were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and by immunoblotting and indirect immunofluorescence assay (IFA) with five to nine monoclonal antibodies (MAbs). By SDS-PAGE results and reactivities to MAbs H3TS, J 8.3, I 17.3, and D6, the 37 isolates were assign...
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We have read with interest the article by Zhibang et al. (12) about an outbreak of infections due to Mycobacterium abscessus. We believe, as the authors did, that the strains isolated from the different patients and the environmental samples were the same clone. Classical epidemiology data support this fact, as did conventional microbiology data and plasmid analysis (although only one plasmid w...
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We describe the resolution of the plasma apolipoproteins with molecular weights from 8,800 to greater than 550,000, using a 3.5% sodium dodecyl sulfate-glycerol polyacrylamide slab gel system. The simplicity of this system and the resolution of proteins over a broad range of molecular weights will make it particularly useful in investigations of apolipoprotein composition of plasma lipoproteins.
متن کاملA recommendation for visualizing disulfide bonding by one-dimensional sodium dodecyl sulfate--polyacrylamide gel electrophoresis.
A simple modification of the conventional one-dimensional sodium dodecyl sulfate--polyacrylamide gel electrophoresis technique has been used to visualize inter- and intramolecular disulfide bonding in proteins. The gradient of reducing agent established between adjacent slab gel tracks by electrophoresing identical protein samples next to one another, one containing and the other not containing...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1982
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.16.2.240-244.1982